PrimePCR™ Probe Assay: CDC16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDC16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDC16, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051558
Assay Design:   exonic
Chromosome Location:   13:115037752-115037898question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000356221 ENST00000375310 ENST00000252457 ENST00000375308 ENST00000252458 ENST00000360383 ENST00000375312

Gene Information

This gene encodes a component protein of the APC complex which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins CDC23 and CDC27 contain a tetratricopeptide repeat (TPR) a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC16
Gene Name:   cell division cycle 16 homolog (S. cerevisiae)
Aliases:   ANAPC6, APC6, CUT9
RefSeq:   NC_000013.10 NT_024498.12
Ensembl:   ENSG00000130177
Entrez:   8881
Chromosome Mapping:   13q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.010000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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