PrimePCR™ Probe Assay: LSM7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LSM7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LSM7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051567
Assay Design:   exonic
Chromosome Location:   19:2321684-2321780question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000400676 ENST00000399451 ENST00000412318 ENST00000383777 ENST00000254323 ENST00000590508 ENST00000440752 ENST00000248923 ENST00000412658 ENST00000400382 ENST00000400380 ENST00000400383 ENST00000326775 ENST00000451158 ENST00000394170 ENST00000326742 ENST00000402119 ENST00000248041 ENST00000378215 ENST00000380243 ENST00000424317 ENST00000328737 ENST00000282272 ENST00000450567 ENST00000337207 ENST00000521015 ENST00000256398 ENST00000524103 ENST00000523357 ENST00000380353 ENST00000537665 ENST

Gene Information

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles which are important for pre-mRNA splicing.[supplied by OMIM Apr 2004]

Gene Symbol:   LSM7
Gene Name:   LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Aliases:   YNL147W
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000130332
Entrez:   51690
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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