PrimePCR™ Probe Assay: BMP15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BMP15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BMP15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051572
Assay Design:   exonic
Chromosome Location:   X:50658782-50658911question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000398249 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000370602 ENST00000395358 ENST00000252677 ENST00000263196 ENST00000537045 ENST00000545799 ENST00000421422 ENST00000373263 ENST00000540170 ENST00000332496 ENST00000411539 ENST00000345136 ENST00000357649 ENST00000354589 ENST00000398774 ENST00000322810 ENST00000354958 ENST00000356346 ENST00000436759 ENST00000402157 ENST00000299550 ENST00000303329 ENST

Gene Information

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.[provided by RefSeq Sep 2009]

Gene Symbol:   BMP15
Gene Name:   bone morphogenetic protein 15
Aliases:   GDF9B, ODG2, POF4
RefSeq:   NC_000023.10 NG_012894.1 NT_011638.13
Ensembl:   ENSG00000130385
Entrez:   9210
Chromosome Mapping:   Xp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.410000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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