This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CYP2E1, Human
PrimePCR™ Template for Probe Assay: CYP2E1, Human
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol the diabetic state and starvation. The enzyme metabolizes both endogenous substrates such as ethanol acetone and acetal as well as exogenous substrates including benzene carbon tetrachloride ethylene glycol and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates this enzyme may be involved in such varied processes as gluconeogenesis hepatic cirrhosis diabetes and cancer. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.