PrimePCR™ Probe Assay: EDA2R, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EDA2R, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EDA2R, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051622
Assay Design:   exonic
Chromosome Location:   X:65819596-65819704question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000374719 ENST00000253392 ENST00000396050 ENST00000456230 ENST00000450752 ENST00000451436

Gene Information

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair sweat glands and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq May 2011]

Gene Symbol:   EDA2R
Gene Name:   ectodysplasin A2 receptor
Aliases:   EDA-A2R, EDAA2R, TNFRSF27, XEDAR
RefSeq:   NC_000023.10 NG_013271.2 NT_011669.17
Ensembl:   ENSG00000131080
Entrez:   60401
Chromosome Mapping:   Xq12

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