PrimePCR™ Probe Assay: RAMP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAMP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAMP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051651
Assay Design:   exonic
Chromosome Location:   17:40914470-40914692question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000589683 ENST00000588928 ENST00000253796 ENST00000587142 ENST00000588576 ENST00000248121 ENST00000433485 ENST00000239243 ENST00000457820 ENST00000219478 ENST00000360761 ENST00000352832 ENST00000442258 ENST00000502264 ENST00000512389 ENST00000513964 ENST00000514717 ENST00000510366 ENST00000503485 ENST00000507510 ENST00000507336 ENST00000513689 ENST00000507609 ENST00000510115 ENST00000515165 ENST00000514181 ENST00000515765 ENST00000514079 ENST00000507896 ENST00000505165 ENST00000515411 ENST

Gene Information

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR a receptor with seven transmembrane domains can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq Jul 2008]

Gene Symbol:   RAMP2
Gene Name:   receptor (G protein-coupled) activity modifying protein 2
Aliases:   Not Available
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000131477
Entrez:   10266
Chromosome Mapping:   17q12-q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.890000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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