PrimePCR™ Probe Assay: PRMT7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRMT7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRMT7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051710
Assay Design:   exonic
Chromosome Location:   16:68349893-68355353question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000348497 ENST00000441236 ENST00000339507 ENST00000449359 ENST00000569047 ENST00000569571 ENST00000565745 ENST00000566657

Gene Information

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases such as PMT7 using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction RNA transport and RNA splicing (Miranda et al. 2004 [PubMed 15044439]).[supplied by OMIM Mar 2008]

Gene Symbol:   PRMT7
Gene Name:   protein arginine methyltransferase 7
Aliases:   FLJ10640, KIAA1933
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000132600
Entrez:   54496
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.140000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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