PrimePCR™ Probe Assay: TEX13A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TEX13A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: TEX13A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051755
Assay Design:   exonic
Chromosome Location:   X:104463774-104463918question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000302917 ENST00000540567 ENST00000372578 ENST00000372575 ENST00000413579 ENST00000600991

Gene Information

This gene is similar to a mouse gene that is expressed in the testis. [provided by RefSeq Jul 2008]

Gene Symbol:   TEX13A
Gene Name:   testis expressed 13A
Aliases:   MGC131984
RefSeq:   NC_000023.10 NG_012566.1 NG_016253.1 NT_011651.17
Ensembl:   ENSG00000133149
Entrez:   56157
Chromosome Mapping:   Xq22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.580000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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