PrimePCR™ Probe Assay: IMPA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IMPA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IMPA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051768
Assay Design:   exonic
Chromosome Location:   8:82592898-82593736question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000256108 ENST00000311489 ENST00000449740 ENST00000523942 ENST00000519964 ENST00000521360 ENST00000522997 ENST00000518202

Gene Information

This gene encodes an enyzme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol a precursor of phosphatidylinositol and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 145-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-13-diphosphate myo-inositol-14-diphosphate scyllo-inositol-phosphate glucose-1-phosphate glucose-6-phosphate fructose-1-phosphate beta-glycerophosphate and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq Nov 2009]

Gene Symbol:   IMPA1
Gene Name:   inositol(myo)-1(or 4)-monophosphatase 1
Aliases:   IMP, IMPA
RefSeq:   NC_000008.10 NG_015829.1 NT_008183.19
Ensembl:   ENSG00000133731
Entrez:   3612
Chromosome Mapping:   8q21.13-q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.860000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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