PrimePCR™ Probe Assay: HSD17B4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSD17B4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSD17B4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051774
Assay Design:   exonic
Chromosome Location:   5:118814615-118824902question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000256216 ENST00000515320 ENST00000510025 ENST00000504811 ENST00000414835 ENST00000513628 ENST00000509514

Gene Information

The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq Jul 2008]

Gene Symbol:   HSD17B4
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 4
Aliases:   DBP, MFE-2, MPF-2, SDR8C1
RefSeq:   NC_000005.9 NG_008182.1 NT_034772.6
Ensembl:   ENSG00000133835
Entrez:   3295
Chromosome Mapping:   5q21

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