PrimePCR™ Probe Assay: EIF2S1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EIF2S1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF2S1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051782
Assay Design:   exonic
Chromosome Location:   14:67843170-67843304question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000256383 ENST00000557310 ENST00000466499 ENST00000555876

Gene Information

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA EIF2 and GTP. EIF2 is composed of 3 nonidentical subunits the 36-kD EIF2-alpha subunit (EIF2S1) the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908) and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. 1987 [PubMed 2948954]).[supplied by OMIM Feb 2010]

Gene Symbol:   EIF2S1
Gene Name:   eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Aliases:   EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000134001
Entrez:   1965
Chromosome Mapping:   14q23.3

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