PrimePCR™ Probe Assay: LOXL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LOXL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LOXL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051784
Assay Design:   exonic
Chromosome Location:   8:23225805-23225904question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000342907 ENST00000393640 ENST00000276833 ENST00000301305 ENST00000280562 ENST00000542749 ENST00000320201 ENST00000392428 ENST00000400337 ENST00000355480 ENST00000342220 ENST00000359759 ENST00000565606 ENST00000569401 ENST00000276420 ENST00000544659 ENST00000328300 ENST00000361603 ENST00000332408 ENST00000303143 ENST00000397799 ENST00000481785 ENST00000416372 ENST00000043402 ENST00000425986 ENST00000260356 ENST00000307980 ENST00000291503 ENST00000357324 ENST00000374423 ENST00000326764 ENST

Gene Information

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation senescence tumor suppression cell growth control and chemotaxis to each member of the family. [provided by RefSeq Jul 2008]

Gene Symbol:   LOXL2
Gene Name:   lysyl oxidase-like 2
Aliases:   LOR2, WS9-14
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000134013
Entrez:   4017
Chromosome Mapping:   8p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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