PrimePCR™ Probe Assay: FBXO18, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FBXO18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FBXO18, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051815
Assay Design:   exonic
Chromosome Location:   10:5958259-5958368question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000362091 ENST00000379999 ENST00000379994 ENST00000397269

Gene Information

This gene encodes a member of the F-box protein family members of which are characterized by an approximately 40 amino acid motif the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box) which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains Fbls containing leucine-rich repeats and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   FBXO18
Gene Name:   F-box protein, helicase, 18
Aliases:   FBH1, FLJ14590, Fbx18, MGC131916, MGC141935, MGC141937
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000134452
Entrez:   84893
Chromosome Mapping:   10p15.1

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