PrimePCR™ Probe Assay: IL2RA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL2RA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL2RA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051817
Assay Design:   exonic
Chromosome Location:   10:6066290-6067860question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000379959 ENST00000379954 ENST00000256876 ENST00000447847

Gene Information

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains together with the common gamma chain (IL2RG) constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq Nov 2009]

Gene Symbol:   IL2RA
Gene Name:   interleukin 2 receptor, alpha
Aliases:   CD25, IDDM10, IL2R, TCGFR
RefSeq:   NC_000010.10 NG_007403.1 NT_008705.16
Ensembl:   ENSG00000134460
Entrez:   3559
Chromosome Mapping:   10p15-p14

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