PrimePCR™ Probe Assay: ERCC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ERCC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ERCC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051839
Assay Design:   exonic
Chromosome Location:   13:103525672-103527779question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000355739 ENST00000375954

Gene Information

This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV-induced damage. The protein may also function in other cellular processes including RNA polymerase II transcription and transcription-coupled DNA repair. Mutations in this gene cause xeroderma pigmentosum complementation group G (XP-G) which is also referred to as xeroderma pigmentosum VII (XP7) a skin disorder characterized by hypersensitivity to UV light and increased susceptibility for skin cancer development following UV exposure. Some patients also develop Cockayne syndrome which is characterized by severe growth defects mental retardation and cachexia. Read-through transcription exists between this gene and the neighboring upstream BIVM (basic immunoglobulin-like variable motif containing) gene. [provided by RefSeq Feb 2011]

Gene Symbol:   ERCC5
Gene Name:   excision repair cross-complementing rodent repair deficiency, complementation group 5
Aliases:   COFS3, ERCM2, UVDR, XPG, XPGC
RefSeq:   NC_000013.10 NG_007146.1 NT_009952.14
Ensembl:   ENSG00000134899
Entrez:   2073
Chromosome Mapping:   13q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.500000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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