PrimePCR™ Probe Assay: PHF21A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PHF21A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PHF21A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051861
Assay Design:   exonic
Chromosome Location:   11:46105734-46105835question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000323180 ENST00000418153 ENST00000524497 ENST00000531959 ENST00000529734 ENST00000529782 ENST00000533757 ENST00000525438 ENST00000527782 ENST00000532010 ENST00000257821

Gene Information

The PHF21A gene encodes BHC80 a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al. 2002 [PubMed 12032298]).[supplied by OMIM Nov 2010]

Gene Symbol:   PHF21A
Gene Name:   PHD finger protein 21A
Aliases:   BHC80, KIAA1696
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000135365
Entrez:   51317
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.060000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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