PrimePCR™ Probe Assay: HTR2B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HTR2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HTR2B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051904
Assay Design:   exonic
Chromosome Location:   2:231978471-231978620question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000258400

Gene Information

This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter a hormone and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution designated Q20*) which blocks expression of this protein and knockout studies in mice suggest a role for this gene in impulsivity. However other factors such as elevated testosterone levels may also be involved. [provided by RefSeq Oct 2011]

Gene Symbol:   HTR2B
Gene Name:   5-hydroxytryptamine (serotonin) receptor 2B
Aliases:   5-HT(2B), 5-HT2B
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000135914
Entrez:   3357
Chromosome Mapping:   2q36.3-q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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