PrimePCR™ Probe Assay: WNT10A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WNT10A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WNT10A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051908
Assay Design:   exonic
Chromosome Location:   2:219747044-219747145question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000258411 ENST00000458582

Gene Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition it and another family member the WNT6 gene are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq Jul 2008]

Gene Symbol:   WNT10A
Gene Name:   wingless-type MMTV integration site family, member 10A
Aliases:   FLJ14301, SSPS
RefSeq:   NC_000002.11 NG_012179.1 NT_005403.17
Ensembl:   ENSG00000135925
Entrez:   80326
Chromosome Mapping:   2q35

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