PrimePCR™ Probe Assay: SETDB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SETDB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SETDB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051934
Assay Design:   exonic
Chromosome Location:   13:50050834-50050983question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000317257 ENST00000258672 ENST00000354234

Gene Information

Proteins that contain a SET domain such as SETDB2 modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al. 2003 [PubMed 12754510]).[supplied by OMIM Mar 2008]

Gene Symbol:   SETDB2
Gene Name:   SET domain, bifurcated 2
Aliases:   C13orf4, CLLD8, CLLL8, DKFZp586I0123, DKFZp761J1217, KMT1F
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000136169
Entrez:   83852
Chromosome Mapping:   13q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.960000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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