PrimePCR™ Probe Assay: PPIL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052014
Assay Design:   exonic
Chromosome Location:   6:36823461-36823597question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000373699

Gene Information

This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved ubiquitous family members of which play an important role in protein folding immunosuppression by cyclosporin A and infection of HIV-1 virions. Based on similarity to other PPIases this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq Jul 2008]

Gene Symbol:   PPIL1
Gene Name:   peptidylprolyl isomerase (cyclophilin)-like 1
Aliases:   CYPL1, MGC678, PPIase, hCyPX
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000137168
Entrez:   51645
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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