PrimePCR™ Probe Assay: ABCG5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ABCG5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ABCG5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052075
Assay Design:   exonic
Chromosome Location:   2:44040070-44040186question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000260645 ENST00000405322 ENST00000543989

Gene Information

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver colon and intestine. This gene is tandemly arrayed on chromosome 2 in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis and have been observed in patients with sitosterolemia. [provided by RefSeq Jul 2008]

Gene Symbol:   ABCG5
Gene Name:   ATP-binding cassette, sub-family G (WHITE), member 5
Aliases:   STSL
RefSeq:   NC_000002.11 NG_008883.1 NG_008884.1 NT_022184.15
Ensembl:   ENSG00000138075
Entrez:   64240
Chromosome Mapping:   2p21

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