PrimePCR™ Probe Assay: DUSP5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DUSP5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DUSP5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052087
Assay Design:   exonic
Chromosome Location:   10:112270835-112270980question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000369583

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1 is expressed in a variety of tissues with the highest levels in pancreas and brain and is localized in the nucleus. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP5
Gene Name:   dual specificity phosphatase 5
Aliases:   DUSP, HVH3
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000138166
Entrez:   1847
Chromosome Mapping:   10q25

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