PrimePCR™ Probe Assay: NCOA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCOA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCOA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052092
Assay Design:   exonic
Chromosome Location:   10:51584799-51584895question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000374087 ENST00000344348 ENST00000374082 ENST00000431200 ENST00000438493 ENST00000452682 ENST00000443446 ENST00000430396 ENST00000414907 ENST00000577819 ENST00000581486 ENST00000585132 ENST00000579039 ENST00000578454 ENST00000583565 ENST00000585056 ENST00000580070 ENST00000578680

Gene Information

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene also located on chromosome 10 have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4 5 10 and 14. [provided by RefSeq Feb 2009]

Gene Symbol:   NCOA4
Gene Name:   nuclear receptor coactivator 4
Aliases:   ARA70, DKFZp762E1112, ELE1, PTC3, RFG
RefSeq:   NC_000010.10 NG_023372.1 NT_030059.13
Ensembl:   ENSG00000138293
Entrez:   8031
Chromosome Mapping:   10q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.820000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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