PrimePCR™ Probe Assay: CHRNA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHRNA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHRNA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052104
Assay Design:   exonic
Chromosome Location:   2:175618267-175618392question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000348749 ENST00000261007 ENST00000409542 ENST00000409219 ENST00000409323

Gene Information

The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha isoforms and 1 each of beta gamma and delta subunits.2 This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   CHRNA1
Gene Name:   cholinergic receptor, nicotinic, alpha 1 (muscle)
Aliases:   ACHRA, ACHRD, CHRNA, CMS2A, FCCMS, SCCMS
RefSeq:   NC_000002.11 NT_005403.17 NG_008172.1
Ensembl:   ENSG00000138435
Entrez:   1134
Chromosome Mapping:   2q24-q32

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