PrimePCR™ Probe Assay: PPCDC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPCDC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPCDC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052110
Assay Design:   exonic
Chromosome Location:   15:75340918-75341025question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000342932 ENST00000564923 ENST00000569562 ENST00000568649 ENST00000567336 ENST00000563393 ENST00000278878 ENST00000378488 ENST00000498508 ENST00000366958 ENST00000435016 ENST00000261454

Gene Information

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36) one of the last enzymes in this pathway converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al. 2002 [PubMed 11923312]).[supplied by OMIM Mar 2008]

Gene Symbol:   PPCDC
Gene Name:   phosphopantothenoylcysteine decarboxylase
Aliases:   FLJ14585
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000138621
Entrez:   60490
Chromosome Mapping:   15q24.2

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