PrimePCR™ Probe Assay: CXCL9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CXCL9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CXCL9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052123
Assay Design:   exonic
Chromosome Location:   4:76924230-76924378question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000264888

Gene Information

The function of this gene has not been specifically defined; however it is thought to be involved in T cell trafficking. This gene has been localized to 4q21 with INP10 which is also a member of the chemokine family of cytokines. [provided by RefSeq Jul 2008]

Gene Symbol:   CXCL9
Gene Name:   chemokine (C-X-C motif) ligand 9
Aliases:   CMK, Humig, MIG, SCYB9, crg-10
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138755
Entrez:   4283
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.520000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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