PrimePCR™ Probe Assay: PRICKLE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRICKLE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRICKLE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052154
Assay Design:   exonic
Chromosome Location:   12:42866235-42877563question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000455697 ENST00000547113 ENST00000551050 ENST00000552108 ENST00000552240 ENST00000345127 ENST00000548696 ENST00000445766

Gene Information

This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq Sep 2009]

Gene Symbol:   PRICKLE1
Gene Name:   prickle homolog 1 (Drosophila)
Aliases:   EPM1B, FLJ31627, FLJ31937, MGC138902, MGC138903, RILP
RefSeq:   NC_000012.11 NT_029419.12 NG_012965.1
Ensembl:   ENSG00000139174
Entrez:   144165
Chromosome Mapping:   12q12

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