PrimePCR™ Probe Assay: ADAD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ADAD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADAD2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052247
Assay Design:   exonic
Chromosome Location:   16:84228711-84228885question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000374568 ENST00000374572 ENST00000395114 ENST00000552779 ENST00000412144 ENST00000383198 ENST00000383197 ENST00000550915 ENST00000548408 ENST00000374719 ENST00000253392 ENST00000396050 ENST00000456230 ENST00000450752 ENST00000451436 ENST00000377294 ENST00000423974 ENST00000434618 ENST00000475304 ENST00000426633 ENST00000456592 ENST00000268624 ENST00000315906 ENST00000567685 ENST00000437315 ENST00000417059 ENST00000456807 ENST00000549949 ENST00000383067 ENST00000383066 ENST00000383065 ENST

Gene Information

Description Not Available

Gene Symbol:   ADAD2
Gene Name:   adenosine deaminase domain containing 2
Aliases:   FLJ00337, TENRL
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140955
Entrez:   161931
Chromosome Mapping:   16q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 37.880000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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