PrimePCR™ Probe Assay: CYP4B1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052344
Assay Design:   exonic
Chromosome Location:   1:47279163-47279267question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000371923 ENST00000271153 ENST00000371919 ENST00000526297 ENST00000452782 ENST00000468637

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents the homologous protein has been shown to metabolize certain carcinogens; however the specific function of the human protein has not been determined. Two transcript variants encoding slightly different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP4B1
Gene Name:   cytochrome P450, family 4, subfamily B, polypeptide 1
Aliases:   CYPIVB1, P-450HP
RefSeq:   NC_000001.10 NG_007939.1 NT_032977.9
Ensembl:   ENSG00000142973
Entrez:   1580
Chromosome Mapping:   1p34-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.490000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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