PrimePCR™ Probe Assay: VPS54, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VPS54, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VPS54, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052431
Assay Design:   exonic
Chromosome Location:   2:64189284-64189427question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000409558 ENST00000272322 ENST00000354504

Gene Information

This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq Jul 2008]

Gene Symbol:   VPS54
Gene Name:   vacuolar protein sorting 54 homolog (S. cerevisiae)
Aliases:   HCC8, SLP-8p, VPS54L, hVps54L
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000143952
Entrez:   51542
Chromosome Mapping:   2p13-p14

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