PrimePCR™ Probe Assay: NAT8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052437
Assay Design:   exonic
Chromosome Location:   2:73867975-73868090question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000272425

Gene Information

This gene isolated using the differential display method to detect tissue-specific genes is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq Sep 2008]

Gene Symbol:   NAT8
Gene Name:   N-acetyltransferase 8 (GCN5-related, putative)
Aliases:   CML1, GLA, Hcml1, TSC501, TSC510
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000144035
Entrez:   9027
Chromosome Mapping:   2p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.950000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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