PrimePCR™ Probe Assay: NR1I2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NR1I2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NR1I2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052491
Assay Design:   exonic
Chromosome Location:   3:119536593-119536718question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000393716 ENST00000466380 ENST00000337940

Gene Information

This gene product belongs to the nuclear receptor superfamily members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4 binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4 including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms some of which use non-AUG (CUG) translation initiation codon have been described for this gene. Additional transcript variants exist however they have not been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   NR1I2
Gene Name:   nuclear receptor subfamily 1, group I, member 2
Aliases:   BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR
RefSeq:   NC_000003.11 NG_011856.1 NT_005612.16
Ensembl:   ENSG00000144852
Entrez:   8856
Chromosome Mapping:   3q12-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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