PrimePCR™ Probe Assay: MANF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MANF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MANF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052504
Assay Design:   exonic
Chromosome Location:   3:51426344-51426483question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000566546 ENST00000205890 ENST00000418233 ENST00000391745 ENST00000552699 ENST00000267115 ENST00000549385 ENST00000395006 ENST00000547798 ENST00000423828 ENST00000528157 ENST00000425354 ENST00000394426 ENST00000327550 ENST00000252992 ENST00000451429 ENST00000574138 ENST00000571761 ENST00000571164

Gene Information

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region including a specific mutation that changes the previously numbered codon 50 from ATG to AGG have been reported in a variety of solid tumors; however these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. [provided by RefSeq Jun 2010]

Gene Symbol:   MANF
Gene Name:   mesencephalic astrocyte-derived neurotrophic factor
Aliases:   ARMET, ARP, MGC142148, MGC142150
RefSeq:   NC_000003.11 NG_012652.2 NG_028012.1 NT_022517.18 NW_003315910.1
Ensembl:   ENSG00000145050
Entrez:   7873
Chromosome Mapping:   3p21.1

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