PrimePCR™ Probe Assay: ZNF673, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: ZNF673, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF673, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052615
Assay Design:   exonic
Chromosome Location:   X:46309894-46322302question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000375231 ENST00000374641 ENST00000223428 ENST00000344302 ENST00000298190 ENST00000377919 ENST00000476762 ENST00000478600 ENST00000487081 ENST00000360017 ENST00000523374 ENST00000414387 ENST00000518708 ENST00000587846 ENST00000187879 ENST00000391960 ENST00000317243 ENST00000591265 ENST00000361362 ENST00000520660 ENST00000520805 ENST00000320129 ENST00000519564 ENST00000404360 ENST00000405858 ENST00000342651 ENST00000394917

Gene Information

This encodes a zinc finger protein with an N-terminal KRAB (Kruppel-associated) domain found in transcriptional repressors. This gene is located in a region of the X chromosome thought to be involved in nonsyndromic X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   ZNF673
Gene Name:   zinc finger family member 673
Aliases:   FLJ20344
RefSeq:   NC_000023.10 NT_079573.4 NG_022935.1
Ensembl:   ENSG00000147121
Entrez:   55634
Chromosome Mapping:   Xp11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.760000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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