PrimePCR™ Probe Assay: TACC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TACC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TACC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052636
Assay Design:   exonic
Chromosome Location:   8:38682870-38684808question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000430309 ENST00000519416 ENST00000520615 ENST00000520340 ENST00000518415 ENST00000521642 ENST00000521050 ENST00000522904 ENST00000317827 ENST00000276520 ENST00000520973 ENST00000521866 ENST00000518809 ENST00000520611 ENST00000443286 ENST00000330691 ENST00000348567 ENST00000379931 ENST00000554719 ENST00000335750

Gene Information

This locus may represent a breast cancer candidate gene. It is located close to FGFR1 on a region of chromosome 8 that is amplified in some breast cancers. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2009]

Gene Symbol:   TACC1
Gene Name:   transforming, acidic coiled-coil containing protein 1
Aliases:   DKFZp686K18126, FLJ42304, Ga55, KIAA1103
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000147526
Entrez:   6867
Chromosome Mapping:   8p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.420000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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