PrimePCR™ Probe Assay: CACNA1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CACNA1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CACNA1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052673
Assay Design:   exonic
Chromosome Location:   9:140997250-141000230question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000277551 ENST00000371372 ENST00000371363 ENST00000371357 ENST00000413253 ENST00000371355 ENST00000277549 ENST00000371365 ENST00000460680 ENST00000296288 ENST00000260645

Gene Information

The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2 beta and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   CACNA1B
Gene Name:   calcium channel, voltage-dependent, N type, alpha 1B subunit
Aliases:   BIII, CACNL1A5, CACNN, Cav2.2
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000148408
Entrez:   774
Chromosome Mapping:   9q34

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