PrimePCR™ Probe Assay: PTPRO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: PTPRO, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPRO, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052783
Assay Design:   exonic
Chromosome Location:   12:15739843-15739982question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000281171 ENST00000348962 ENST00000442921 ENST00000542557 ENST00000445537 ENST00000544244 ENST00000535322

Gene Information

This gene encodes a member of the R3 subtype family of receptor-type protein tyrosine phosphatases. These proteins are localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. This gene contains two distinct promoters and alternatively spliced transcript variants encoding multiple isoforms have been observed. The encoded proteins may have multiple isoform-specific and tissue-specific functions including the regulation of osteoclast production and activity inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor and decreased expression of this gene has been observed in several types of cancer. [provided by RefSeq May 2011]

Gene Symbol:   PTPRO
Gene Name:   protein tyrosine phosphatase, receptor type, O
Aliases:   GLEPP1, PTP-U2, PTP-oc, PTPU2
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000151490
Entrez:   5800
Chromosome Mapping:   12p13.3-p13.2,12p13-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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