PrimePCR™ Probe Assay: TUBA3E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052811
Assay Design:   exonic
Chromosome Location:   2:130955934-130956032question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000550606 ENST00000548791 ENST00000552724 ENST00000551817 ENST00000355413 ENST00000473247 ENST00000535825 ENST00000597551 ENST00000204637 ENST00000600429 ENST00000595510 ENST00000594009 ENST00000597914 ENST00000301488 ENST00000314537 ENST00000398285 ENST00000376718 ENST00000426088 ENST00000428286 ENST00000312988 ENST00000575872 ENST00000573706 ENST00000586755

Gene Information

Description Not Available

Gene Symbol:   TUBA3E
Gene Name:   tubulin, alpha 3e
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000152086
Entrez:   112714
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.540000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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