PrimePCR™ Probe Assay: CAST, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052853
Assay Design:   exonic
Chromosome Location:   5:96100959-96101780question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000338252 ENST00000508830 ENST00000395812 ENST00000510756 ENST00000508608 ENST00000341926 ENST00000511049 ENST00000309190 ENST00000510156 ENST00000509903 ENST00000511782 ENST00000504465 ENST00000510500 ENST00000437034 ENST00000508579 ENST00000515663 ENST00000395813 ENST00000359176 ENST00000325674

Gene Information

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4) and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jun 2010]

Gene Symbol:   CAST
Gene Name:   calpastatin
Aliases:   BS-17, MGC9402
RefSeq:   NC_000005.9 NG_027839.1 NT_034772.6
Ensembl:   ENSG00000153113
Entrez:   831
Chromosome Mapping:   5q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992300
y-intercept 35.510000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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