PrimePCR™ Probe Assay: NEIL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NEIL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NEIL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052894
Assay Design:   exonic
Chromosome Location:   8:11640804-11643478question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000436750 ENST00000455213 ENST00000403422 ENST00000528323 ENST00000284503

Gene Information

NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al. 2002 [PubMed 12509226])[supplied by OMIM Mar 2008]

Gene Symbol:   NEIL2
Gene Name:   nei endonuclease VIII-like 2 (E. coli)
Aliases:   FLJ31644, MGC2832, MGC4505, NEH2, NEI2
RefSeq:   NC_000008.10 NT_077531.4
Ensembl:   ENSG00000154328
Entrez:   252969
Chromosome Mapping:   8p23.1

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