PrimePCR™ Probe Assay: SH3RF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SH3RF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SH3RF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052901
Assay Design:   exonic
Chromosome Location:   4:170190306-170190448question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000284637 ENST00000502315 ENST00000510806

Gene Information

This gene encodes a protein containing an N-terminus RING-finger four SH3 domains and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway facilitating the formation of a functional signaling module. [provided by RefSeq Jul 2008]

Gene Symbol:   SH3RF1
Gene Name:   SH3 domain containing ring finger 1
Aliases:   FLJ21602, KIAA1494, POSH, RNF142, SH3MD2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000154447
Entrez:   57630
Chromosome Mapping:   4q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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