PrimePCR™ Probe Assay: RASA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RASA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RASA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052959
Assay Design:   exonic
Chromosome Location:   3:141327377-141327523question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000286364 ENST00000452898

Gene Information

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS thereby allowing control of cellular proliferation and differentiation. This particular family member has a perinuclear localization and is an inositol 1345-tetrakisphosphate-binding protein; a compound suggested to function as a second messenger. [provided by RefSeq Jul 2008]

Gene Symbol:   RASA2
Gene Name:   RAS p21 protein activator 2
Aliases:   GAP1M
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000155903
Entrez:   5922
Chromosome Mapping:   3q22-q23

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