PrimePCR™ Probe Assay: EEF1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EEF1A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EEF1A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052990
Assay Design:   exonic
Chromosome Location:   6:74229061-74229187question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000316292 ENST00000309268 ENST00000331523 ENST00000356303 ENST00000455918

Gene Information

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain placenta lung liver kidney and pancreas and the other isoform (alpha 2) is expressed in brain heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes some of which if not all represent different pseudogenes. [provided by RefSeq Jul 2008]

Gene Symbol:   EEF1A1
Gene Name:   eukaryotic translation elongation factor 1 alpha 1
Aliases:   CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000156508
Entrez:   1915
Chromosome Mapping:   6q14.1

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