PrimePCR™ Probe Assay: SMG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053023
Assay Design:   exonic
Chromosome Location:   16:18903569-18907378question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000446231 ENST00000565324 ENST00000561947 ENST00000565224 ENST00000532700 ENST00000569122 ENST00000389467

Gene Information

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described but their full-length natures have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   SMG1
Gene Name:   SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Aliases:   61E3.4, ATX, KIAA0421, LIP
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000157106
Entrez:   23049
Chromosome Mapping:   16p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download