PrimePCR™ Probe Assay: EXTL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053057
Assay Design:   exonic
Chromosome Location:   1:26348934-26349059question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000374280

Gene Information

This gene is a member of the multiple exostoses (EXT) family of glycosyltransferases which function in the chain polymerization of heparan sulfate and heparin. The encoded protein harbors alpha 14- N-acetylglucosaminyltransferase activity and is involved in chain elongation of heparan sulfate and possibly heparin. [provided by RefSeq Jul 2008]

Gene Symbol:   EXTL1
Gene Name:   exostoses (multiple)-like 1
Aliases:   EXTL, MGC70794
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000158008
Entrez:   2134
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.430000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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