PrimePCR™ Probe Assay: SLC13A3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: SLC13A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC13A3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053069
Assay Design:   exonic
Chromosome Location:   20:45239085-45242101question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000279027 ENST00000472148 ENST00000495082 ENST00000468915 ENST00000420568 ENST00000417157 ENST00000413164 ENST00000290317 ENST00000396360 ENST00000435032 ENST00000372121 ENST00000339636

Gene Information

Mammalian sodium-dicarboxylate cotransporters transport succinate and other Krebs cycle intermediates. They fall into 2 categories based on their substrate affinity: low affinity and high affinity. Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys. The protein encoded by this gene represents the high-affinity form. Alternatively spliced transcript variants encoding different isoforms have been found for this gene although the full-length nature of some of them have not been characterized yet. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC13A3
Gene Name:   solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
Aliases:   NADC3, SDCT2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000158296
Entrez:   64849
Chromosome Mapping:   20q12-q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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