PrimePCR™ Probe Assay: DEDD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DEDD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DEDD, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053093
Assay Design:   exonic
Chromosome Location:   1:161092075-161092172question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000368006 ENST00000368005 ENST00000392188 ENST00000545495 ENST00000458050

Gene Information

This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF a basal factor required for RNA polymerase I transcription in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DEDD
Gene Name:   death effector domain containing
Aliases:   CASP8IP1, DEDD1, DEFT, FLDED1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000158796
Entrez:   9191
Chromosome Mapping:   1q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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