PrimePCR™ Probe Assay: SYNJ1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SYNJ1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SYNJ1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053100
Assay Design:   exonic
Chromosome Location:   21:34011303-34011395question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000334828 ENST00000530372 ENST00000268720 ENST00000319518 ENST00000550896 ENST00000396425 ENST00000549643 ENST00000438952 ENST00000382499 ENST00000433931 ENST00000418301 ENST00000416083 ENST00000382491 ENST00000322229 ENST00000357345 ENST00000341928 ENST00000540638 ENST00000544123 ENST00000342933 ENST00000400111 ENST00000427684 ENST00000542146 ENST00000264546 ENST00000412892 ENST00000335211 ENST00000166244

Gene Information

This gene encodes a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-45-bisphosphate. As such expression of this enzyme may affect synaptic transmission and membrane trafficking. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   SYNJ1
Gene Name:   synaptojanin 1
Aliases:   INPP5G
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000159082
Entrez:   8867
Chromosome Mapping:   21q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.440000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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