PrimePCR™ Probe Assay: HK3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053180
Assay Design:   exonic
Chromosome Location:   5:176311053-176314010question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000292432 ENST00000514058

Gene Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2 this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq Apr 2009]

Gene Symbol:   HK3
Gene Name:   hexokinase 3 (white cell)
Aliases:   HKIII, HXK3
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000160883
Entrez:   3101
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.140000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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