PrimePCR™ Probe Assay: SQSTM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SQSTM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SQSTM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053186
Assay Design:   exonic
Chromosome Location:   5:179250901-179251016question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000514093 ENST00000422245 ENST00000389805 ENST00000504627 ENST00000510187 ENST00000360718 ENST00000376929 ENST00000402874

Gene Information

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq Mar 2009]

Gene Symbol:   SQSTM1
Gene Name:   sequestosome 1
Aliases:   A170, OSIL, PDB3, ZIP3, p60, p62, p62B
RefSeq:   NC_000005.9 NG_011342.1 NT_023133.13
Ensembl:   ENSG00000161011
Entrez:   8878
Chromosome Mapping:   5q35

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